Journal: Frontiers in Pharmacology

Article Title: Quercitrin Attenuates Acetaminophen-Induced Acute Liver Injury by Maintaining Mitochondrial Complex I Activity

doi: 10.3389/fphar.2021.586010

Figure Lengend Snippet: Effect of quercitrin on APAP-induced inflammation. (A) Effect of quercitrin on F4/80 expression in the liver tissue of mice (200×). (B) IOD of immunohistochemistry. (C,D) Effect of quercitrin on serum IL-6 and TNF-α levels in APAP-induced liver injury mice. The experimental data are expressed as mean ± SD, ## p < 0.01, compared with the control group; ** p < 0.01, compared with the APAP group.

Article Snippet: Interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) ELISA kits were purchased from Elabscience (Wuhan, China).

Techniques: Expressing, Immunohistochemistry, Control

Journal: The Indonesian Biomedical Journal

Article Title: TNFR, TRAF2, NF-κB mRNA Levels of Glioblastoma Multiforme Cells Treated by Conditioned Medium of Umbilical Cord-derived Mesenchymal Stem Cells

doi: 10.18585/inabj.v11i2.722

Figure Lengend Snippet: Figure 5. TNFR2 level protein. Protein sample in the amount of 100 μL was used to measure TNFR2 level using ELISA with sandwich ELISA method. Protein TNFR2 level concentration was measured with absorbance 450 nm. TNFR2 concentration in CM treated T98G cells was 11.57 pg/mg protein while concentration in control was 7.72 pg/mg protein **(p=0.001). All data displayed in mean±SE (n=6).

Article Snippet: 2019; 11(2): 217-24DOI: 10.18585/inabj.v11i2.722 Enzyme Linked Imunoabsorbance Assay (ELISA) of TNF-α and TNFR2 TNF-α level from conditioned medium was measured using sandwich ELISA method with human ELISA TNF-α assay kit (Catalogue #E-EL-H0109, Elabscience, Texas, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Sandwich ELISA, Concentration Assay, Control

Journal: Chinese Medicine

Article Title: OptiSyn: an interpretable, multi-omics–driven graph convolutional network framework for synergy-oriented drug combination design in disease treatment

doi: 10.1186/s13020-026-01385-1

Figure Lengend Snippet: Effects of pharmacological interventions on immune activation, inflammatory responses, and safety profiles in AS mice. Flow cytometric analyses showing the gating strategy used to identify lymphocytes, exclude doublets, select live cells, and quantify A CD80⁺ and ( B ) CD86⁺ cells within the parent population. C Quantitative analysis of the CD80/CD86 ratio across groups. Serum inflammatory cytokine levels of D IL-6 and ( E ) TNF-α were quantified by ELISA. Safety assessments include liver function markers ( F ) aspartate aminotransferase and ( G ) alanine aminotransferase, renal function indices ( H ) creatinine and ( I ) urea nitrogen, and hematological parameters: ( J ) red blood cells, ( K ) white blood cells, ( L ) neutrophil proportion, ( M ) lymphocyte proportion, ( N ) hemoglobin, and ( O ) platelet counts (n = 3). * P < 0.05, ** P < 0.01 vs. Con; # P < 0.05, ## P < 0.01 vs. AS

Article Snippet: Mouse serum was analyzed following the manufacturer’s instructions to determine levels of hematological parameters (red blood cells, white blood cells, hemoglobin; Nanjing Jiancheng, China), liver function markers (ALT, AST; Nanjing Jiancheng, China), kidney function markers (creatinine, blood urea nitrogen; Nanjing Jiancheng, China), and inflammatory cytokines TNF-α (batch: KE00154) and IL-6 (batch: KE00139; Proteintech, China).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay